Journal: Endocrinology

Article Title: TGFβ Superfamily Members Mediate Androgen Deprivation Therapy-Induced Obese Frailty in Male Mice.

doi: 10.1210/en.2016-1580

Figure Lengend Snippet: Figure 7. Castration induces myostatin protein levels in skeletal muscle. A, Representative immunoblots of myostatin and -actin (reprobing of the myostatin blot) expression in GAS muscle. B, Quantification of mean myostatin levels in GAS muscles from 4 mice at each time, assessed by 3 independent measurements. C, Representative immunoblot of myostatin and - actin (reprobing of the myostatin blot) expression level in TRI muscle. Lanes of immunoblots marked (C) contain identical control sample for interblot comparison. D, Quantification of mean myostatin levels in TRI muscles from 4 mice at each time, assessed by 3 independent measurements. The values shown in B and D are relative to the sham-castrated (0-wk castrate) animals. Sham-castrated (blue) and castrated groups (red) were compared by one-way ANOVA with Dunnett’s testing. Bars are SEM; *, P .05; **, P .01; ***, P .001 vs sham-castrated group.

Article Snippet: Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Myostatin Mouse GDF8 Asn25Ser376 MAb (clone 84214) R&D Systems, MAB788 Monoclonal; rat IgG2B 1:1000 Phospho-Smad2 Smad3S423/5 MAb (clone EP823Y) Abcam, 52903 Monoclonal; rabbit 1:2000 Smad2 Amino terminus hSmad2 L16D3 Cell Signaling, 3103 Monoclonal; mouse 1:2000 Eukaryotic EF2 Complete protein G270 Nastiuk (Ref. 63) Rabbit 1:2000 -Actin -Actin aa 1–14 AC15 Sigma, A5441 Monoclonal; mouse 1:5000 Rat IgG (H L) Rabbit antirat unconjugated Pierce, 31218 Rabbit 1:20 000 Rabbit IgG (H L) Goat antirabbit HRP- conjugated secondary Pierce, 31460 Goat 1:20 000 Mouse IgG (H L) Goat antimouse HRPconjugated secondary Pierce, 31430 Goat 1:20 000 D ow nloaded from https://academ ic.oup.com /endo/article/157/11/4461/2758425 by G ITAM (D eem ed to be U niversity) user on 01 January 2025 tion are an acutely sensitive biological measure of circulating androgen elimination (34, 35).

Techniques: Western Blot, Expressing, Muscles, Control, Comparison

Journal: Biomolecules

Article Title: The Influence of Stress and Binge-Patterned Alcohol Drinking on Mouse Skeletal Muscle Protein Synthesis and Degradation Pathways

doi: 10.3390/biom14050527

Figure Lengend Snippet: Changes in the ubiquitin-proteasome pathway and myostatin following acute stress and alcohol access. Both representative blots ( A ) and relative protein expression ( B – E ) are presented. Data are presented as mean ± SEM ( n = 9/group). A two-way ANOVA was used to compare the individual effects of stress exposure and binge-patterned drinking, as well as to compare and interaction between factors (reported next to each figure).

Article Snippet: Therefore, we assessed changes in relative protein expression of the precursor myostatin protein (#sc-134345; Santa Cruz Biotechnology), commonly used to determine myostatin protein expression [ , ].

Techniques: Ubiquitin Proteomics, Expressing

Journal: The Journal of Physical Fitness and Sports Medicine

Article Title: Age-related changes in myostatin expression in rat skeletal muscles

doi: 10.7600/jpfsm.7.221

Figure Lengend Snippet: Fig. 3 Changes in the mean level of myostatin in soleus, PL, EDL, and TA muscles during aging. Values are means ± SE. n = 10 per group. *,†Significant difference between 7 weeks and 1 year groups, respectively (P < 0.05).

Article Snippet: After serial washes with TTBS, the membranes were reacted overnight at 8°C with anti-myostatin antibody (1:100; R&D Systems, AF788) diluted in the blocking buffer.

Techniques: Muscles